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Activation of the alternative complement pathway by Sporothrix schenckii
Infection and Immunity
|January 1, 1986
Summary
Sporothrix schenckii yeast cells activate the alternative complement pathway independently of antibodies. This study provides a foundation for investigating the role of this pathway in host defenses against fungal infections.
Area of Science:
- Immunology
- Mycology
Background:
- Sporothrix schenckii is a fungus causing sporotrichosis.
- The complement system is crucial for innate immunity.
- Understanding fungal complement activation is key to developing treatments.
Purpose of the Study:
- To investigate the mechanism of complement activation by Sporothrix schenckii yeast cells.
- To determine if antibody is required for complement activation by S. schenckii.
Main Methods:
- Assessing total complement activity in human serum with and without chelators.
- Measuring complement component consumption (C3, C4) and deposition (C3, IgG).
- Utilizing immunofluorescence and antibody absorption assays.
Main Results:
- S. schenckii activated complement via the alternative pathway in vitro.
- Complement component C3 was consumed, while C4 was preserved in non-chelated serum.
- C3 deposition was observed on yeast cells, independent of antibody presence.
Conclusions:
- Sporothrix schenckii yeast cells activate the alternative complement pathway independently of antibodies in vitro.
- This activation mechanism warrants further investigation into its role in host defense against S. schenckii infections.