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Updated: May 10, 2026
11:08
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
Published on: June 22, 2012
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Suspended Tissue Open Microfluidic Patterning (STOMP)
Amanda J Haack1,2, Lauren G Brown1, Alex J Goldstein3,4,5
1Department of Chemistry, University of Washington, Seattle, WA, 98195 USA.
Biorxiv : the Preprint Server for Biology
|October 17, 2024
Summary
We developed Suspended Tissue Open Microfluidic Patterning (STOMP) to create multi-region tissues for studying cell contraction at complex interfaces. This method reveals altered contractile dynamics in fibrotic-healthy and bone-ligament tissues.
Area of Science:
- Mechanobiology
- Tissue Engineering
- Biomaterials
Background:
- Free-standing tissues are vital for studying cell contraction.
- Existing methods lack the ability to model complex tissue interfaces.
- There is a need for advanced tools to investigate diseased-healthy and tissue-type boundaries.
Purpose of the Study:
- To develop a novel method for creating multiregional suspended tissues.
- To enable the study of complex tissue interfaces, such as fibrotic-healthy and bone-ligament boundaries.
- To investigate altered contractile dynamics at these interfaces.
Main Methods:
- Suspended Tissue Open Microfluidic Patterning (STOMP) utilizes open microfluidics and capillary pinning.
- STOMP patterns subregions within free-standing tissues.
- This method allows for tissue generation in various geometries using native extracellular matrices and 4D materials.
Main Results:
- STOMP successfully created multiregional suspended tissues.
- Altered contractile dynamics were observed in fibrotic-healthy engineered heart tissues compared to single-region controls.
- Differential contractility was noted in bone-ligament enthesis constructs versus single-tissue periodontal ligament models.
Conclusions:
- STOMP is a versatile platform for generating patterned, free-standing tissues.
- The method facilitates the study of complex tissue interfaces and their unique contractile behaviors.
- STOMP combines suspended tissue functionality with precise patterning for dynamic, spatially controlled mechanobiology research.
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