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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 10, 2025

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

Stimulated Brillouin scattering flow cytometry.

Jake R Rosvold¹, Giulia Zanini¹, Chenchen Handler²

¹Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USA.

Biomedical Optics Express

|October 18, 2024

Summary

We developed a faster method using stimulated Brillouin scattering flow cytometry for rapid cell biomechanics measurement. This technique significantly improves speed for analyzing cell populations, including cancer cells.

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Area of Science:

Biophysics
Cell Biology
Spectroscopy

Background:

Cell biomechanics are crucial for understanding cell function and disease.
Existing methods for measuring cell biomechanics can be slow.
Flow cytometry offers high-throughput analysis but has limitations in speed for certain measurements.

Purpose of the Study:

To introduce and validate a novel stimulated Brillouin scattering flow cytometry (sBS-FC) system.
To demonstrate significantly enhanced measurement speeds for cell biomechanics.
To differentiate between normal and cancerous breast epithelial cells based on biomechanical properties.

Main Methods:

Utilized stimulated Brillouin scattering (sBS) spectroscopy integrated into a flow cytometer.
Achieved high acquisition rates of 200 Hz with rapid spectral acquisition times of 5 ms.
Experimentally measured biomechanical properties of normal breast epithelial cells and metastatic breast cancer cells.

Main Results:

Demonstrated a 10x speed improvement over spontaneous Brillouin scattering flow cytometry.
Successfully differentiated between normal and metastatic breast epithelial cells using sBS-FC.
Validated the capability of sBS-FC for rapid, high-throughput biomechanical analysis.

Conclusions:

Stimulated Brillouin scattering flow cytometry provides a rapid and effective method for cell biomechanics analysis.
This technique offers a significant advancement for high-throughput screening and cell characterization.
sBS-FC has potential applications in cancer research and diagnostics.