Related Experiment Video
Updated: Jun 9, 2025

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
RHO subfamily of small GTPases in the development and function of hematopoietic cells
Stephany Suelen de Castro Sampaio1, Maria Carolina Clares Ramalho1, Caroline Santos de Souza1
1Department of Clinical and Experimental Oncology, Federal University of São Paulo, São Paulo, Brazil.
None:
RHOA, RHOB, and RHOC comprise a subfamily of RHO GTPase proteins famed for controlling cytoskeletal dynamics. RHO proteins operate downstream of multiple signals emerging from the microenvironment, leading to diverse cell responses, such as proliferation, adhesion, and migration. Therefore, RHO signaling has been centrally placed in the regulation of blood cells. Despite their high homology, unique roles of RHOA, RHOB, and RHOC have been described in hematopoietic cells. In this article, we overview the contribution of RHO proteins in the development and function of each blood cell lineage. Additionally, we highlight the aberrations of the RHO signaling pathways found in hematological malignancies, providing clues for the identification of new therapeutic targets.
Related Concept Videos
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Role of Hematopoietic Growth Factors
Thrombopoietin (TPO), mainly released by the liver,...
Cell Polarization by Rho Proteins
GTPases and their Regulation
Large G-proteins,...
Activation and Inactivation of G Proteins
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...

