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Updated: Jun 12, 2025
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Published on: December 21, 2019
Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration
Juhi Chakraborty1, Chandrashish Roy1, Maria Kalogeropoulou2
1Department of Textile and Fibre Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
Abstract:
External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stem/stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration. For complete details on the use and execution of this protocol, please refer to Chakraborty et al.1.
