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Updated: Jun 9, 2025

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
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Synergistic Inhibition of Colorectal Cancer Cells by Autocrine Motility Factor Peptide and Glycyrrhetinic Acid

Se Gie Kim1, Thanh Van Duong2, Semin Lee3

  • 1Department of Cosmetic Science, Kyungsung University, 48434 Busan, Republic of Korea.

Discovery Medicine
|October 28, 2024
PubMed
Abstract

Insights

This study shows that autocrine motility factor (AMF) peptides combined with glycyrrhetinic acid (GA) from licorice plants synergistically inhibit colorectal cancer (CRC) cell growth and colony formation by increasing reactive oxygen species (ROS). This suggests a potential new peptide-phytochemical therapy for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Anti-cancer peptides offer broad efficacy by inducing cancer cell death.
  • Autocrine motility factor (AMF) regulates cancer cell processes.
  • Synergistic effects of plant-derived anti-cancer compounds and AMF are understudied.

Purpose of the Study:

  • To evaluate AMF-derived peptides' effects on colorectal cancer (CRC) cells.
  • To investigate the synergistic impact of AMF peptides combined with glycyrrhetinic acid (GA) on CRC.

Main Methods:

  • Assessed AMF peptides' effects on CRC cell proliferation, colony formation, NADP+/NADPH ratio, and ROS generation.
  • Investigated combined AMF peptides and GA effects on CRC cell proliferation, colony formation, ROS production, and cell cycle progression.

Main Results:

  • AMF peptides significantly inhibited CRC cell growth and colony formation (p < 0.05).
  • AMF peptides increased the NADP+/NADPH ratio and ROS production (p < 0.05 and p < 0.001, respectively).
  • Combined AMF peptides and GA enhanced GA's anti-cancer effects, further suppressing growth and colony formation while increasing ROS (p < 0.05).

Conclusions:

  • AMF peptides demonstrate anti-cancer properties against CRC cells.
  • Synergy observed between AMF peptides and GA suggests potential for combined peptide-phytochemical therapy in CRC treatment.
Keywords:
autocrine motility factor peptidecolorectal cancerglycyrrhetinic acidreactive oxygen species

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