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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

Long-range enhancement for fluorescence and Raman spectroscopy using Ag nanoislands protected with column-structured

Takeo Minamikawa^1,2,3,4, Reiko Sakaguchi⁵, Yoshinori Harada⁶

¹Department of Systems Innovation, Graduate School of Engineering Science, Osaka University, Osaka, 560-8531, Japan. minamikawa.takeo.es@osaka-u.ac.jp.

Light, Science & Applications

|October 28, 2024

Summary

We developed remote plasmonic-like enhancement (RPE) plates using silver nanoislands and silica coatings. These plates significantly boost fluorescence and Raman scattering signals for biosensing and tissue imaging without direct analyte contact.

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Area of Science:

Plasmonics
Nanotechnology
Spectroscopy

Background:

Conventional plasmonic enhancement relies on near-field interactions between analytes and metal nanostructures.
Existing methods often require close proximity between the analyte and the nanostructure, limiting applications.

Purpose of the Study:

To demonstrate a novel remote plasmonic-like enhancement (RPE) effect for fluorescence and Raman scattering.
To develop a high-productivity fabrication method for RPE plates.
To evaluate the application of RPE in biosensing and histological imaging.

Main Methods:

Fabrication of dense random arrays of silver nanoislands (AgNIs) coated with column-structured silica (CSS) overlayer.
Utilizing sputtering and chemical immersion in gold(I)/halide solution for RPE plate production.
Applying RPE plates for fluorescence biosensing in HeLa cells and Raman histological imaging of oesophagus tissues.

Main Results:

Achieved long-range enhancement of fluorescence (10^2-fold) and Raman scattering (10^7-fold).
Demonstrated successful application in intracellular signalling dynamics monitoring and tissue imaging.
Observed enhancement factors that deviate from conventional near-field enhancement theories.

Conclusions:

RPE plates offer significant signal enhancement for Raman scattering and fluorescence without analyte proximity.
The technology shows promise for advanced biosensing and biomolecular analysis in various biological and medical fields.
Further research into the underlying mechanisms of RPE is warranted, but practical advantages are evident.