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Related Concept Videos

Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Gene Regulation in Microbial Communities: Quorum Sensing01:28

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Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
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Prokaryotic Transcriptional Activators and Repressors01:58

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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Light inducible gene expression system for Streptomyces.

Ryuta Noya1, Kyohei Murakoshi1, Madoka Fukuda1

  • 1Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-0880, Japan.

Scientific Reports
|October 29, 2024
PubMed
Summary

Researchers developed a light-inducible system in Streptomyces bacteria using LitR and LitS regulators. This system enables hyperproduction of enzymes and secondary metabolites, offering new possibilities for microbial biotechnology.

Keywords:
StreptomycesLight-inducibleLitROptogeneticsRecombinant protein

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Area of Science:

  • Microbiology
  • Synthetic Biology
  • Biotechnology

Background:

  • The LitR/CarH family are photosensory regulators controlling light-inducible carotenoid production in bacteria.
  • Nonphototrophic bacteria utilize these systems for light-dependent metabolic processes.

Purpose of the Study:

  • To establish a blue-green light-inducible hyperexpression system in Streptomyces griseus using LitR and LitS.
  • To demonstrate the system's capability for hyperproduction of various molecules.

Main Methods:

  • Constructed a multi-copy plasmid (pLit19) containing key genetic elements for light-inducible expression.
  • Introduced pLit19 into Streptomyces griseus and Streptomyces sp. NBRC 13304.
  • Tested light sensitivity by inserting a sti fragment and using shuttle-type plasmids.

Main Results:

  • Streptomyces transformants showed light-dependent hyper-production of intracellular and extracellular enzymes.
  • Light-inducible production of secondary metabolites like melanin and actinorhodin was observed.
  • Enhanced light sensitivity was achieved, enabling expression under weak light.

Conclusions:

  • Successfully established an optogenetically controlled hyperproduction system for Streptomyces.
  • The developed system allows precise control over gene expression using light.
  • This system has potential applications in microbial biotechnology and secondary metabolite production.