Benzothiazole-Based Fluorescent Probe as a Simple and Effective Platform for Functional Mitochondria Imaging
Kyeong-Im Hong1, Younghun Kim1, Jeong Heon Lee1
1Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea.
Chemical & Biomedical Imaging
|October 30, 2024
Summary
Researchers developed a novel hydroxythiophene-conjugated benzothiazole platform for creating advanced fluorescent probes. These probes enable functional mitochondrial imaging by detecting esterase activity and pH changes linked to mitochondrial dysfunction.
Area of Science:
- Mitochondrial imaging
- Fluorescent probe development
- Bioimaging chemistry
Background:
- Mitochondrial imaging is vital for disease research but limited by available probes.
- Existing cationic probes are difficult to modify for functional bioimaging.
Purpose of the Study:
- To design a versatile platform (BzT-OH) for novel functional fluorescent probes.
- To synthesize and evaluate BzT-OAc and BzT-OAcryl probes for mitochondrial bioimaging.
Main Methods:
- Synthesis of BzT-OH, BzT-OAc, and BzT-OAcryl.
- Evaluation of probe fluorescence properties and enzymatic activity detection.
- Assessment of pH-responsive detection and biocompatibility in mitochondria.
Main Results:
- BzT-OAc detects endogenous esterase activity via fluorescence shift.
- BzT-OAcryl selectively detects cysteine and pH changes in mitochondria.
- Both probes show high biocompatibility, quantum yield, and large Stokes shifts.
Conclusions:
- The BzT-OH platform facilitates the design of functional fluorescent probes for mitochondrial imaging.
- These probes offer new tools for studying mitochondrial function and disease.
- The hydroxyl group's role in excited state intermolecular proton transfer influences photofunctional properties.
