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Updated: Jun 12, 2025
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
Published on: October 10, 2015
Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived
Yuri Atsumi1, Nobuhiko Yamamoto2, Noriyuki Sugo1
1Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
Abstract:
Single-molecule imaging (SMI) is a powerful approach to quantify the spatiotemporal dynamics of transcription in living cells. Here, we describe a protocol of SMI for transcription and epigenetic factors in human cortical neurons derived from embryonic stem cells or induced pluripotent stem cells. Specifically, we detail the procedures for neural stem cell culture, gene transfer, microscopy, and data analysis. This protocol can be applied to the study of transcription dynamics in response to various cellular stimuli. For complete details on the use and execution of this protocol, please refer to Atsumi et al.1.
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