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Single gold nanowire-nanoparticles conjugated system: Fabrication and sensing application
Qingshan Dai1, Yanyan Zhu1, Xianzhun Luo1
1Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, PR China.
Researchers developed a novel single gold nanowire and gold-platinum nanoparticle system for sensitive thrombin detection using surface-enhanced Raman spectroscopy (SERS). This platform offers real-time analysis with high selectivity and a low detection limit.
Area of Science:
- Nanotechnology
- Biosensing
- Analytical Chemistry
Background:
- Single-entity sensing platforms enable real-time assays and single-molecule analysis.
- Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for sensitive detection.
Purpose of the Study:
- To develop a novel single gold nanowire (Au NW) - gold-platinum/gold nanoparticle (Au@Pt/Au NPs) conjugated system.
- To utilize this system for sensitive and selective detection of thrombin using SERS.
Main Methods:
- Fabrication of a single Au NW - Au@Pt/Au NPs conjugated system.
- Utilizing electrostatic interactions between thrombin aptamer and crystal violet (CV) for SERS signal modulation.
- Monitoring changes in SERS signals upon thrombin binding to the aptamer.
Main Results:
- The system demonstrated a decrease in SERS signals due to reduced CV adsorption, which was recovered upon thrombin detection.
- Achieved high sensitivity and selectivity for thrombin sensing.
- Established a linear range of 0.01 nM-100 nM with a detection limit of 1.35 pM for thrombin.
Conclusions:
- The single Au NW - Au@Pt/Au NPs conjugation offers enhanced SERS hotspots and a small size for superior sensing.
- This platform is advantageous for real-time analysis, particularly at the single molecule or cell level.
