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Updated: Jun 8, 2025

Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and Swallowing
Published on: December 1, 2023
A first step towards objective grading of equine laryngeal function.
Josephine Hardwick1, Benjamin Ahern2, Samantha Franklin3
1School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia 5317, Australia; School of Veterinary Medicine, Murdoch University, Murdoch, Western Australia 6150, Australia.
A new diagnostic decision tree (DDT) standardizes equine laryngeal function (LF) grading by objectively defining terms. This tool aims to reduce veterinarian disagreement in assessing LF, improving diagnostic consistency.
Area of Science:
- Veterinary Medicine
- Equine Science
- Diagnostic Imaging
Background:
- Veterinary disagreement in grading equine laryngeal function (LF) necessitates standardized diagnostic methods.
- Existing grading systems can be ambiguous, leading to inconsistent assessments.
Purpose of the Study:
- To develop and validate a diagnostic decision tree (DDT) for objective grading of resting equine laryngeal function (LF).
- To reduce ambiguity and improve consistency in LF grading among veterinarians.
Main Methods:
- Codified the Havemeyer grading system into a flowchart-based DDT.
- Used videoendoscopic examinations with unanimous LF grades from experienced observers to develop the DDT.
- Defined objective cut-off values for key parameters like left arytenoid cartilage (LAC) abduction duration and angle.
Main Results:
- The DDT provides a series of yes/no decisions to guide LF grade determination.
- Objective definitions were established for maintained full LAC abduction (≥0.2s) and partial abduction (≥45° vs <45°).
- The DDT offers a structured approach to LF assessment, potentially aiding computer-assisted grading.
Conclusions:
- The developed DDT offers a standardized, objective method for grading equine laryngeal function.
- Implementation of the DDT can enhance diagnostic consistency and reduce inter-observer variability.
- This tool serves as a foundational step towards automated laryngeal function assessment in horses.
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