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¹³C NMR: ¹H–¹³C Decoupling01:04

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Unlocking Single-Particle Multiparametric Sensing: Decoupling Temperature and Viscosity Readouts through Upconverting

Elisa Ortiz-Rivero1,2, Katarzyna Prorok3, Riccardo Marin1,2,4

  • 1Nanomaterials for Bioimaging Group, Departamento de Física de Materiales, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, 28049, Spain.

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|November 4, 2024
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Summary
This summary is machine-generated.

This study demonstrates how a single spinning upconverting particle (UCP) can simultaneously measure temperature and viscosity. This breakthrough overcomes crosstalk issues for accurate multiparametric sensing in microenvironments.

Keywords:
optical trappingrheometersingle‐particle thermometerupconverting particles

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Spectroscopy

Background:

  • Upconverting particles (UCPs) convert infrared to visible light, useful for imaging and sensing.
  • UCPs can act as remote multiparametric sensors but suffer from crosstalk, hindering accurate measurements.
  • Crosstalk occurs when different stimuli cause similar luminescence changes, leading to interpretation errors.

Purpose of the Study:

  • To demonstrate simultaneous and independent sensing of temperature and viscosity using a single spinning UCP.
  • To overcome the challenge of crosstalk in multiparametric UCP sensing.
  • To validate a novel approach for unbiased sensing in microenvironments.

Main Methods:

  • Utilized a spinning NaYF4:Er3+, Yb3+ upconverting particle.
  • Employed luminescence from thermally coupled energy levels of Er3+ ions for temperature sensing.
  • Leveraged luminescence polarization from non-thermally coupled levels of Er3+ ions for viscosity sensing.

Main Results:

  • Achieved simultaneous and independent readings of temperature and viscosity from a single UCP.
  • Successfully decoupled thermal and rheological measurements.
  • Validated the unbiased sensing capability through proof-of-concept experiments.

Conclusions:

  • A single spinning UCP can perform simultaneous temperature and viscosity sensing.
  • This method effectively overcomes crosstalk limitations in UCP-based multiparametric sensing.
  • Opens new possibilities for advanced sensing applications in microscale environments.