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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Updated: Jun 8, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

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Multi-species cryoEM calibration and workflow verification standard.

Daija Bobe¹, Mykhailo Kopylov¹, Jessalyn Miller¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.

Acta Crystallographica. Section F, Structural Biology Communications

|November 4, 2024

Summary

A new cryo-electron microscopy (cryoEM) calibration sample with compatible macromolecules is introduced. This standard aids in optimizing resolution, evaluating instrument performance, and validating cryoEM workflows.

Keywords:

3D reconstruction and image processing benchmarking calibration cryo-electron microscopy education resolution single-particle analysis single-particle cryoEM structure determination training workflow standard

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Last Updated: Jun 8, 2025

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Published on: July 12, 2022

Area of Science:

Structural biology
Biophysics

Background:

Cryogenic electron microscopy (cryoEM) is a powerful technique for determining molecular structures.
Established techniques often use calibration standards, but cryoEM lacks comprehensive biological test samples.

Purpose of the Study:

To introduce a novel cryo-EM calibration sample.
To provide a standardized method for optimizing resolution and evaluating cryo-EM performance.

Main Methods:

Development of a cryo-EM calibration sample comprising a mixture of compatible macromolecules.
Utilizing the sample for resolution optimization and performance evaluation.

Main Results:

The calibration sample enables resolution optimization.
It provides multiple reference points for assessing instrument performance, data quality, and image-processing workflows.
The sample serves as a validation tool for cryo-EM pipelines and methodologies.

Conclusions:

The developed cryo-EM calibration sample addresses the need for standardized biological test specimens.
This resource facilitates benchmarking and the testing of new algorithms in cryo-EM research.