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Characterization and complete genome sequence of highly lytic phage active against methicillin-resistant

Abeer K Abd El-Tawab1, B A Othman2, A Sharaf3,4

  • 1Department of Agriculture Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. abeerkourany@agr.asu.edu.eg.

Virology Journal
|November 9, 2024
PubMed
Abstract

Insights

A novel lytic phage, vB_SauP_ASUmrsa123, was identified against Methicillin-Resistant Staphylococcus aureus (MRSA) and its genome sequenced. This phage shows potential as a safe and effective alternative to antibiotics for treating MRSA infections.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriology

Background:

  • Methicillin-Resistant Staphylococcus aureus (MRSA) poses a significant threat due to antibiotic resistance.
  • Bacteriophages are explored as natural, specific, and effective alternatives to antibiotics for MRSA infections.

Purpose of the Study:

  • To characterize a newly isolated lytic phage, vB_SauP_ASUmrsa123.
  • To evaluate its potential for controlling MRSA infections.

Main Methods:

  • Isolation and confirmation of MRSA strains.
  • Phage isolation from environmental and clinical samples.
  • Transmission electron microscopy for morphology and whole-genome sequencing.

Main Results:

  • A lytic phage, vB_SauP_ASUmrsa123, was isolated and confirmed against 25 MRSA clinical isolates.
  • Morphological analysis placed the phage in the Rountreeviridae family.
  • Genome sequencing revealed a 17,155 bp linear dsDNA with 24 ORFs.

Conclusions:

  • The characterized lytic phage vB_SauP_ASUmrsa123 is effective against MRSA.
  • Its genome sequencing provides insights into staphylococcal phages.
  • The phage is a promising candidate for biocontrol applications.
Keywords:
Staphylococcus aureusAntimicrobial resistance (AMR)Genome sequencingMRSAPhage

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