Related Experiment Video
Updated: Jun 7, 2025
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
Published on: March 30, 2017
Live Imaging of Fission Yeast Single-Cell Lineages Using a Microfluidic Device
1Department of Optical Imaging, Advanced Research Promotion Center, Tokushima University, Tokushima, Japan. nakaoka.hidenori@tokushima-u.ac.jp.
Abstract:
Mother machine (MM) is a microfluidic device originally developed for long-term live imaging of Escherichia coli bacterial cells under a microscope. The simple yet sophisticated design has enabled microbiologists to track multiple single-cell lineages cultured under highly controlled external environments. Here, I describe how to fabricate a fission yeast version of MM with photolithography and soft lithography. Procedures for setting up the microfluidic device for long-term live microscopy are also explained.
