Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Drug-tolerant persisters to TRAIL emerge from a dose-dependent surface in a cell-state continuum of sensitivity.

NPJ systems biology and applications·2026

Same author

Circadian clock model with sequestration repression motif: existence of periodic orbits and entrainment properties.

Interface focus·2025

Same author

Analytic solutions for the circadian oscillator characterize cycle dynamics and its robustness.

Journal of mathematical biology·2024

Same author

[Harmonic oscillations of circadian rhythms come out of the shadows].

Medecine sciences : M/S·2023

Same author

Cycle dynamics and synchronization in a coupled network of peripheral circadian clocks.

Interface focus·2022

Same author

Two-level modeling approach to identify the regulatory dynamics capturing drug response heterogeneity in single-cells.

Scientific reports·2021

Search research articles

Related Experiment Video

Updated: Jun 7, 2025

A Computational Method to Quantify Fly Circadian Activity

A Computational Method to Quantify Fly Circadian Activity

Published on: October 28, 2017

Benchmark for quantitative characterization of circadian clock cycles.

Odile Burckard¹, Michèle Teboul², Franck Delaunay²

¹Centre Inria d'Université Côte d'Azur, INRAE, CNRS, Macbes team, Sophia Antipolis, France.

|November 17, 2024

Summary

This study introduces a new method to analyze circadian clock cycles by segmenting them into eight distinct stages. This approach helps compare mathematical models and experimental data, improving our understanding of circadian rhythms and related diseases.

Keywords:

Algorithm for circadian cycle characterization Circadian clock Computational method Cycle segmentation into stages Model comparison

More Related Videos

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

Related Experiment Videos

Last Updated: Jun 7, 2025

A Computational Method to Quantify Fly Circadian Activity

A Computational Method to Quantify Fly Circadian Activity

Published on: October 28, 2017

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Published on: November 11, 2016

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

Area of Science:

Chronobiology
Systems Biology
Mathematical Biology

Background:

Circadian clock mechanisms are crucial for health, and their malfunctioning is linked to diseases.
Mathematical models are vital for understanding the dynamics of circadian oscillators.
Quantitative comparison of these models and experimental data is challenging.

Purpose of the Study:

To develop a quantitative method for analyzing and comparing circadian cycles.
To establish a standardized approach for evaluating mathematical models of circadian rhythms.
To provide insights into the effects of genetic perturbations on circadian dynamics.

Main Methods:

Proposed a method based on circadian cycle segmentation into a sequence of eight stages.
Applied the segmentation approach to an experimental dataset.
Validated the method using five different ordinary differential equation models of circadian oscillators.

Main Results:

Successfully segmented circadian cycles into eight characteristic stages.
Demonstrated the method's ability to assess the agreement between mathematical models and biological properties.
Identified inconsistencies in mathematical models and provided insights into gene function effects.

Conclusions:

The proposed stage-based segmentation offers a robust benchmark for characterizing and comparing circadian models.
This method enhances the analysis of circadian dynamics and aids in the improvement of mathematical models.
Facilitates a deeper understanding of circadian clock function and its role in health and disease.