Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrinsic cell rheology drives junction maturation.

Nature communications·2022
Same author

Release of insulin granules by simultaneous, high-speed correlative SICM-FCM.

Journal of microscopy·2020
Same author

Planar Airy beam light-sheet for two-photon microscopy.

Biomedical optics express·2020
Same author

Microtubules regulate cardiomyocyte transversal Young's modulus.

Proceedings of the National Academy of Sciences of the United States of America·2020
Same author

Stem Cell Expansion and Fate Decision on Liquid Substrates Are Regulated by Self-Assembled Nanosheets.

ACS nano·2018
Same author

Light-sheet microscopy with attenuation-compensated propagation-invariant beams.

Science advances·2018
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K

Widefield optical coherence tomography by electro-optical modulation.

Dorian R Urban1,2,3, Pavel Novak1, Miguel A Preciado1

  • 1Optos PLC, Queensferry House, Enterprise Way, Dunfermline KY11 8GR, Scotland, UK.

Biomedical Optics Express
|November 18, 2024
PubMed
Summary
This summary is machine-generated.

Optical coherence tomography (OCT) overcomes depth limitations for imaging curved samples like retinas. Harmonic OCT synthesizes images at any depth without mechanical adjustments, enhancing widefield retinal imaging.

More Related Videos

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

2.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.2K

Related Experiment Videos

Last Updated: May 7, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

2.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.2K

Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Optical Engineering

Background:

  • Optical coherence tomography (OCT) offers high-resolution, micron-level axial sectioning for various applications, including ophthalmology.
  • Widefield retinal imaging is crucial for diagnosing retinal diseases, but fast OCT systems face depth-range limitations due to sample curvature and scan speed trade-offs.
  • Current swept-source OCT technologies, while fast, struggle with limited depth range, hindering real-time imaging of curved biological tissues.

Purpose of the Study:

  • To develop a method for extending the effective depth range of OCT systems for real-time imaging of highly curved samples.
  • To enable widefield retinal imaging at high scan speeds without mechanical repositioning.
  • To overcome the inherent trade-off between scan speed and depth range in OCT.

Main Methods:

  • Utilized opto-electronic modulation of a single-frequency swept source laser.
  • Implemented tailored numerical dispersion compensation techniques.
  • Demonstrated harmonic image synthesis at any depth without mechanical sample manipulation.

Main Results:

  • Achieved an 8-fold extension of the effective depth range for real-time imaging of highly curved samples.
  • Successfully enabled widefield retinal imaging even at a 400 kHz swept source scan speed.
  • Synthesized harmonic images at any depth, overcoming field-of-view restrictions.

Conclusions:

  • Harmonic OCT effectively extends the depth range, enabling real-time widefield imaging of curved samples like the retina.
  • The developed opto-electronic modulation and numerical compensation method overcomes limitations of current fast OCT systems.
  • This advancement is significant for clinical ophthalmology and other fields requiring high-resolution imaging of curved surfaces.