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Updated: Jun 7, 2025

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

ImmunoCellCycle-ID - a high-precision immunofluorescence-based method for cell cycle identification.

Yu-Lin Chen¹, Yu-Chia Chen^1,2, Aussie Suzuki^1,2,3

¹McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.

Journal of Cell Science

|November 20, 2024

Summary

This summary is machine-generated.

A new immunofluorescence method, ImmunoCellCycle-ID, precisely identifies cell cycle stages at the single-cell level. This user-friendly technique offers detailed cell cycle analysis as an alternative to flow cytometry.

Keywords:

Cell cycle Imaging Immunofluorescence-based cell cycle identification Microscopy

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Last Updated: Jun 7, 2025

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Published on: December 20, 2014

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Published on: December 16, 2014

Area of Science:

Cell Biology
Molecular Biology

Background:

The cell cycle, comprising G1, S, G2, and M phases, is vital for cell proliferation and differentiation.
Proteins regulating the cell cycle are critical in cancer prevention and progression.
Traditional methods like flow cytometry require large cell numbers for accurate cell cycle analysis.

Purpose of the Study:

To develop a user-friendly, single-cell resolution method for precise cell cycle stage identification.
To offer an alternative or complementary approach to flow cytometry for detailed cell cycle analysis.

Main Methods:

Development of an immunofluorescence-based assay named ImmunoCellCycle-ID.
Utilizing fluorescence microscopy for precise identification of G1, S, G2, and M phases, including sub-stages.

Main Results:

ImmunoCellCycle-ID provides single-cell resolution for cell cycle stage identification.
The method accurately distinguishes between G1, early/mid S, late S, early/mid G2, late G2, and M phase sub-stages.
High-precision cell cycle identification was achieved across various cell lines.

Conclusions:

ImmunoCellCycle-ID is a valuable tool for detailed cell cycle analysis at the single-cell level.
This method can complement or replace traditional flow cytometry for dissecting cell cycle sub-stages.
The technique enhances the study of cell cycle regulation in diverse cell types.