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Modeling of Isoproterenol-Induced Chronic Heart Failure in 24-Month-Old Rats
T Yu Rebrova1, V A Korepanov2, I V Stepanov2
1Cardiology Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia. rebrova@cardio-tomsk.ru.
Bulletin of Experimental Biology and Medicine
|November 21, 2024
Summary
This study successfully modeled chronic heart failure (CHF) in aged rats using isoproterenol hydrochloride injections. The method induced characteristic CHF changes and is suitable for further research into heart failure in older animal models.
Area of Science:
- Cardiovascular Science
- Animal Models of Disease
- Pharmacology
Background:
- Chronic heart failure (CHF) is a significant health concern, particularly in aging populations.
- Reliable animal models are crucial for understanding CHF pathogenesis and testing interventions.
Purpose of the Study:
- To establish and validate a model of chronic heart failure (CHF) in aged Wistar rats.
- To assess the feasibility of using isoproterenol hydrochloride (ISO) for inducing CHF in this specific age group.
Main Methods:
- Male Wistar rats (24 months old) received four subcutaneous injections of isoproterenol hydrochloride (ISO) (10 mg/kg) or saline at 3-day intervals.
- Animals were observed for 60 days post-injection, with mortality and histological changes assessed.
Main Results:
- A 30% mortality rate was observed in the ISO-induced CHF group.
- Histological examination revealed myocardial, kidney, and lung alterations consistent with CHF.
- The ISO administration protocol effectively induced chronic heart failure in aged rats.
Conclusions:
- The described isoproterenol hydrochloride administration scheme provides a viable model for chronic heart failure in 24-month-old rats.
- This model can be utilized for investigating age-related cardiovascular diseases and therapeutic strategies.
