Related Experiment Video
Updated: Jun 6, 2025
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Quantification of Neutralizing Antibodies in Serum Using VSV-MARV-GFP
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA. paige.fletcher@nih.gov.
Abstract:
Neutralizing antibodies play an important role in protection against filoviruses. However, the technique to assess neutralization titers with a replicating BSL-4 virus is limited. This chapter explains how to determine the neutralization titer in serum samples against Marburg virus (MARV). This method uses a replicating recombinant vesicular stomatitis virus expressing the MARV glycoprotein and the green fluorescent protein as a surrogate approach.
