Related Experiment Video
Updated: Jun 6, 2025
08:40
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
4.2K
Studying Structural Details in Complex Samples: II. High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS)
Alessandro Vetere1, Wolfgang Schrader1
1Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, D-45470 Mülheim (Ruhr), Germany.
Journal of the American Society for Mass Spectrometry
|November 25, 2024
Summary
Analyzing complex mixtures like crude oil requires advanced techniques. This study combines ion mobility separation with ultrahigh resolution mass spectrometry to identify structural motifs in complex samples.
Area of Science:
- Analytical Chemistry
- Petroleum Chemistry
Background:
- Structural elucidation of complex mixtures is challenging with standard methods.
- Ultrahigh resolution mass spectrometry (UHR-MS) alone lacks detailed structural information.
- Chromatography struggles with isomer separation in complex mixtures.
Purpose of the Study:
- To evaluate the combination of ion mobility separation and UHR-MS for analyzing complex mixtures.
- To develop analytical techniques for crude oil, a highly complex natural sample.
- To elucidate structural motifs of crude oil constituents and isomers.
Main Methods:
- Utilized a lab-built atmospheric pressure photoionization/atmospheric pressure laser ionization-field asymmetric ion mobility spectrometry (APPI/APLI-FAIMS) source.
- Optimized ion transmission for improved analysis of crude oil components.
- Employed tandem mass spectrometry (MS/MS) with collision-induced dissociation (CID) for structural analysis.
Main Results:
- Successfully coupled ion mobility separation with UHR-MS for complex mixture analysis.
- Demonstrated the capability to separate and analyze isomers within crude oil.
- Obtained insights into the structural motifs of photoionized crude oil constituents.
Conclusions:
- The combination of ion mobility separation and UHR-MS is effective for analyzing complex mixtures.
- This approach enhances the structural elucidation of isomers, particularly in challenging matrices like crude oil.
- The optimized APPI/APLI-FAIMS source and MS/MS strategy provide valuable data on molecular structures.
Related Concept Videos
01:21Mass Spectrometry: Complex Analysis
723
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
723
01:15High-Resolution Mass Spectrometry (HRMS)
1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
01:19Mass Analyzers: Common Types
570
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
570
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
01:19Mass Spectrometry: Overview
4.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
4.6K
01:13Mass Analyzers: Overview
596
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
596