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Updated: Jun 6, 2025
Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
Published on: November 14, 2018
In-situ oxidative polymerization of dopamine triggered by CuO2 in acidic condition and application in "turn-off"
Yang Huang1, Shangming Wu1, Siyi Yang1
1Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, PR China.
Abstract:
Dopamine (DA) is a key neurotransmitter whose concentration affects various neurological disorders. Unlike previous methods that synthesize non-fluorescent polydopamine (NFL-PDA) under alkaline conditions, this study introduces a novel "turn-off" sensing method for DA using NFL-PDA synthesized through a unique reaction pathway. In our approach, CuO2 nanodots, created via a simple reduction method, catalyze the formation of hydroxyl radicals (•OH) in acidic conditions, triggering the oxidative polymerization of DA into NFL-PDA. The reaction between DA and CuO2 nanodots in acidic solution was examined to understand the process. UiO-66-NH2 was then used to test NFL-PDA's fluorescence quenching ability and to further investigate the DA determination mechanism. Results showed that fluorescence quenching was due to enhanced non-radiative energy transfer and Förster resonance energy transfer (FRET) between NFL-PDA and UiO-66-NH2. This led to the development of a simple "turn-off" fluorometric DA determination method with a linear range of 0.1-200 μmol/L and a limit of detection (LOD) of 0.0575 μmol/L. Although this method does not outperform existing NFL-PDA synthesis methods under alkaline conditions, it provides a new synthesis approach and application for sensing DA, contributing to the basic theory of chemical sensing. Additionally, DA determination in human serum samples was successfully achieved, with results consistent with high-performance liquid chromatography (HPLC).
