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Comparative Study of Non-invasive Mouse Models of Pancreatitis
Kamatam Swetha1, Mylanayakanahosahalli Chandrashekar Indumathi1, Shiva Siddappa2
1Department of Studies in Biochemistry, University of Mysore, Manasagangotri, Mysore-06, India.
Digestive Diseases and Sciences
|November 28, 2024
Summary
The caerulein+lipopolysaccharide (LPS) model is the most reliable for inducing pancreatitis in mice. This study compared three models, finding caerulein+LPS most effective for pancreatitis research.
Area of Science:
- Gastroenterology
- Animal Models
- Pancreatic Diseases
Background:
- Establishing a reliable mouse model for pancreatitis is crucial for disease research.
- Existing non-invasive models have significant limitations.
Purpose of the Study:
- To compare the efficiency, consistency, and reproducibility of three non-invasive mouse models for inducing pancreatitis.
- To identify the most suitable animal model for pancreatitis research.
Main Methods:
- Evaluated L-arginine-induced, caerulein-induced, and caerulein+lipopolysaccharide (LPS)-induced pancreatitis models in Wistar albino mice.
- Assessed model efficiency through enzyme levels (amylase, lipase) and histological analysis.
- Administered L-arginine (4 g/kg) or caerulein (50 µg/kg) via intraperitoneal injections, with LPS (10 mg/kg) used in combination.
Main Results:
- The L-arginine model demonstrated inconsistency in inducing pancreatitis.
- Caerulein and caerulein+LPS models showed elevated pancreatic enzyme levels.
- Histological analysis revealed tissue destruction, with fibrosis observed in the caerulein+LPS group.
Conclusions:
- The caerulein+LPS model is the most reliable for inducing pancreatitis in Wistar albino mice.
- This study provides valuable insights for selecting appropriate animal models in pancreatitis research.
