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Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii
Patricia Orlandi Barth1, Dariane Castro Pereira2, Gabriela Simões de Oliveira3
1Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil; Programa de Pós-Graduação em Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Background:
Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.
Methods:
All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC, blaGES, blaOXA-48-like, and blaOXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.
Results:
Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the blaNDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the blaNDM-1 was carried by the same plasmid type.
Discussion:
Our study has identified a novel strain of A seifertii carrying blaNDM-1 responsible for the outbreak, indicating its emergence in the institution.
Conclusions:
This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.
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