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Moldable Alginate/Hydroxyapatite Hydrogel Loaded with Metformin Enhanced Regeneration of the Rabbit Mandibular
Sara S Nabavizadeh1, Mehri Shadi2, Mohammadhossein Khorraminejad-Shirazi3,4,5
1Department of Otolaryngology, Otolaryngology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Journal of Maxillofacial and Oral Surgery
|December 2, 2024
Summary
Metformin-loaded alginate/hydroxyapatite hydrogel effectively repairs rabbit mandibular defects. This biocompatible hydrogel enhances bone formation, showing synergistic effects with hydroxyapatite for critical-sized bone regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Moldable hydrogels loaded with osteoinductive agents like metformin show promise for bone defect reconstruction.
- Alginate/hydroxyapatite hydrogels offer a suitable matrix for delivering metformin to enhance bone repair.
Purpose of the Study:
- To evaluate metformin-loaded alginate/hydroxyapatite hydrogel for accelerating critical-sized mandibular defect repair in rabbits.
- To assess the biocompatibility and osteogenic potential of the hydrogel in vitro and in vivo.
Main Methods:
- In vitro: Cytotoxicity and osteoinduction of metformin-loaded hydrogel assessed using MG63 cells.
- In vivo: Rabbit mandibular defects treated with alginate/hydroxyapatite hydrogel (with or without metformin) and analyzed via CT and histomorphometry.
Main Results:
- Hydrogel scaffolds were non-cytotoxic and promoted cell osteogenesis (alkaline phosphatase activity, calcium deposition).
- In vivo, metformin-treated groups showed enhanced osteogenesis, increased bone-specific cells, and reduced scaffold remnants.
- CT scans confirmed superior bone filling in defects treated with alginate/hydroxyapatite/metformin hydrogel.
Conclusions:
- Locally delivered metformin in alginate/hydroxyapatite hydrogel demonstrates biocompatibility and osteogenesis properties.
- The study highlights a synergistic effect between metformin and hydroxyapatite in promoting bone regeneration.
