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Updated: Aug 5, 2026

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Published on: February 9, 2009
Orthopoxvirus Purification for High Containment Laboratories
Laura Hughes1, Kimberly Wilkins2, Scott Smith2
1Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. bkz2@cdc.gov.
Abstract:
Purification of large viruses in a high containment laboratory can create unique challenges. Traditional purification methods for large viruses rely on equipment and techniques that are not ideal for high containment work. Poxvirus purification has long relied on the use of Genetron®, a reagent that is no longer available. Here we describe a purification protocol that is effective for semi-purification of orthopoxviruses and suitable for work in high containment laboratories.
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