High-Throughput Single-Cell Analysis of Vaccinia Virus Infection

Here, we describe a robust and flexible method for analyzing the infection of single cells with a fluorescent-reporter virus, with real-time tracking of infected cells through microscopy. We subsequently generate quantitative data from the resulting time-lapse microscopy, and harness these data to generate biologically meaningful parameters at scale. Our methodology offers a practical solution for researchers seeking to understand the complexities and variability of virus-host interactions at a granular level.