Limosilactobacillus Fermentun ZS09 Can Improve Antibiotic-Induced Motor Dysfunction in Mice by Regulating the...

Area of Science:

• Microbiology and Neuroscience
• Gut Microbiome Research
• Probiotic Therapeutics

Background:

• Probiotics, live microorganisms, offer health benefits when consumed appropriately.
• Antibiotic use can disrupt gut microbiota, potentially leading to motor impairments.
• Limosilactobacillus fermentum ZS09 (LFZS09) is a probiotic strain with potential therapeutic applications.

Purpose of the Study:

• To investigate the preventive effects of Limosilactobacillus fermentum ZS09 (LFZS09) on antibiotic-induced dyskinesia in a mouse model.
• To assess the impact of LFZS09 on motor function, gut microbiota, and neuroinflammation in mice.

Main Methods:

• Establishment of a mouse model of antibiotic-induced dyskinesia.
• Assessment of motor function (running, swimming), oxidative stress markers (MDA, SOD), and inflammatory cytokines (IL-6, TNF-α) in serum and brain tissue.
• Evaluation of intestinal barrier gene expression (occludin-1, ZO-1, claudin-1) in cecal tissue and neurotrophic factor pathway gene expression (CREB, ERK, BDNF) in brain tissue.
• Analysis of gut microbiota composition (Lactobacillus, Bifidobacterium, Enterococcus, Clostridium perfringens).

Main Results:

• LFZS09 significantly improved motor function, increasing running and swimming duration in mice.
• LFZS09 reduced levels of inflammatory factors (TNF-α, IL-6) and oxidative stress markers (MDA) while increasing SOD expression.
• LFZS09 upregulated intestinal barrier genes, enhanced BDNF pathway gene expression, and modulated gut microbiota composition, increasing beneficial bacteria and decreasing harmful ones.

Conclusions:

• LFZS09 demonstrates a significant preventive effect against antibiotic-induced motor impairment in mice.
• LFZS09 acts by maintaining intestinal barrier integrity, enhancing neurotrophic support, and rebalancing gut microbiota.
• These findings provide a theoretical basis for developing LFZS09-based probiotics for augmenting motor function.