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Ultrasmall High-Entropy-Alloy Nanozyme Catalyzed In Vivo ROS and NO Scavenging for Anti-Inflammatory Therapy
Daeeun Choi1, Yeonju Boo2, Seonhye Park1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Advanced Healthcare Materials
|December 6, 2024
Summary
High-entropy alloy (HEA) nanozymes mimic biological redox systems. These novel nanoparticles show enhanced catalytic activity and superior anti-inflammatory effects in vitro and in vivo.
Area of Science:
- Nanotechnology
- Materials Science
- Biochemistry
Background:
- High-entropy alloy (HEA) nanoparticles offer tunable catalytic activity due to diverse adsorption sites.
- Mimicking biological redox homeostasis, involving complex enzymatic reactions, is crucial but underexplored for HEAs.
- Regulating biological redox reactions is vital for maintaining cellular health.
Purpose of the Study:
- To synthesize ultra-small HEA nanozymes with tunable morphologies.
- To investigate the catalytic activity of HEA nanozymes compared to monometallic nanoparticles.
- To evaluate the efficacy of HEA nanozymes in mimicking biological redox reactions and their anti-inflammatory potential.
Main Methods:
- Synthesis of ultra-small (<10 nm) HEA nanozymes using five platinum-group metals.
- Tuning nanoparticle morphologies from planar to dendritic structures.
- Assessing peroxidase-like activity, mimicking reactive oxygen and nitrogen species (RONS) metabolism, and evaluating in vitro/in vivo anti-inflammatory effects.
Main Results:
- Synthesized HEA nanozymes exhibited higher peroxidase-like activity than monometallic platinum-group nanoparticles.
- HEA nanoparticles successfully mimicked cascade reactions (superoxide dismutase, catalase) and multiple reactions (HORAC, NO scavenging).
- The HEA nanozyme demonstrated superior anti-inflammatory efficacy in both in vitro and in vivo models.
Conclusions:
- HEA nanozymes effectively restore in vivo enzymatic systems by enhancing intrinsic activity.
- The high-entropy alloy structure facilitates cascade and multiple reaction mechanisms for biological redox regulation.
- These findings highlight the potential of HEA nanozymes as therapeutic agents for inflammatory conditions.
