Layered Double Hydroxide (LDH)-Based Nanotripod for High-Entropydynamic Therapy Associated with Metabolism...

Area of Science:

• Biomedical Engineering
• Materials Science
• Nanotechnology

Background:

• Multielemental transition metal compounds show promise for biomedical applications.
• Synthesizing these compounds often requires challenging high-temperature treatments.
• Developing novel agents for advanced therapies like high-entropy-dynamic therapy is crucial.

Purpose of the Study:

• To synthesize a novel high-entropy oxide (HEO) nanotripod agent for high-entropy-dynamic therapy.
• To investigate the agent's ability to generate reactive oxygen species (ROS) and disrupt tumor metabolism.
• To evaluate the agent's potential for magnetic resonance imaging (MRI)-guided therapy.

Main Methods:

• Fabrication of HEO nanotripod agent via topotactic transformation of layered double hydroxide (LDH) precursors.
• Assessment of ROS generation efficiency regulated by structural disorder.
• Evaluation of the agent's effect on intracellular metabolic balance (NADH/NAD+) and NO-like activity.
• In vitro and in vivo experiments for therapeutic efficacy and MRI guidance.

Main Results:

• Successfully synthesized HEO nanotripod agent with tunable disorder.
• Demonstrated highly efficient ROS generation and regulation via structural turbulence.
• Disrupted tumor cell metabolism by affecting the NADH/NAD+ cycle and exhibiting NO-like activity, leading to apoptosis.
• Confirmed the agent's suitability for MRI-guided therapy in both in vitro and in vivo models.

Conclusions:

• The developed HEO nanotripod is a novel and effective agent for high-entropy-dynamic therapy.
• This agent offers a promising strategy for cancer treatment by inducing metabolic imbalance and apoptosis.
• The findings highlight a new approach for developing advanced therapeutic agents with imaging capabilities.