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Updated: Jun 5, 2025

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol
Published on: April 2, 2018
Open-Flask Protocol for the Removal of Alloc Carbamate and Allyl Ester Protecting Groups. Application to In-solution
Patrick Napier1, Nicole Bakas1, Abhijit Bhat1
1RayzeBio, Inc, 5505 Morehouse Drive, Suite 300, San Diego, California 92121, United States.
Abstract:
The standard protocol for Alloc group removal during peptide synthesis still presents limitations, including low reaction yields, N-allylated byproducts, and the use of air-sensitive Pd(PPh3)4. We addressed these challenges by developing a novel protocol using the air-stable Pd(PPh3)2Cl2 catalyst, Meldrum's acid (MA), and triethylsilane (TES-H). This combination ensured high yields, eliminated N-allylated byproducts, and is compatible with automated synthesis. This cost-effective and efficient method shows promise for widespread use in peptide chemistry applications and beyond.
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