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In vitro bioactive dentin protein release by diode laser conditioning.
Claudia García-Guerrero1, Ana Priscila Rodas Serrano1, Maria Carolina Leal Fernández2
1Departamento de Ciencias Básicas y Medicina Oral, Facultad de Odontología, Universidad Nacional de Colombia, Grupo de Investigación INVENDO, Bogotá, Colombia.
Summary
Ethylenediaminetetraacetic acid (EDTA) combined with diode lasers can enhance the release of transforming growth factor-beta 1 (TGF-β1) from root dentin. Low-level diode lasers alone have limited protein release capabilities.
Area of Science:
- Biomaterials Science
- Dental Research
- Laser Dentistry
Background:
- Root canal treatment aims to remove irritants and seal the canal space.
- Growth factors within dentin matrix play a role in tissue regeneration.
- Ethylenediaminetetracetic acid (EDTA) is commonly used for smear layer removal.
Purpose of the Study:
- To investigate the release of key growth factors from root dentin.
- To evaluate the effect of diode laser application, with and without EDTA, on growth factor release.
- To quantify transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF)-BB, and vascular endothelial growth factor (VEGF).
Main Methods:
- In-vitro study using 30 hemi-roots.
- Groups included Tris-buffered solution (TBS), 17% EDTA, and diode laser (650nm or 810nm) with or without EDTA.
- Growth factor concentrations measured using cytokine bead array.
Main Results:
- TGF-β1 and VEGF were released in all tested protocols.
- Low-level diode laser power demonstrated limited protein release from the dentin matrix.
- No significant difference in VEGF and PDGF-BB release was observed between laser groups.
- A synergistic effect between EDTA and diode laser significantly increased TGF-β1 release.
Conclusions:
- EDTA in conjunction with diode laser enhances TGF-β1 release from root dentin.
- Diode lasers alone have minimal impact on growth factor release.
- Further research is needed to explore the regenerative potential of EDTA-enhanced growth factor release.
