A single-parameter analog level discriminator: a cheap and powerful extension of FCM data acquisition

Modern flow cytometers offer the possibility to measure more parameters of individual cells than the normally available data acquisition equipment can store. To enlarge the number of parameters that is taken into account while recording data, an analog level discriminator is described that is easy to build and easy to implement on any existing FCM system. An example of gated data acquisition in light scattering measurements on nucleated human bone marrow cells is given. The level discriminator may give a cheap and yet valuable extension of expensive data acquisition equipment.