High-performance liquid chromatographic method for determination of voriconazole in pure and gel formulation

For routine measurement of Voriconazole (VZ) in a pure and gel formulation, a quick and accurate RP-HPLC technique with UV detection (254 nm) was developed. With a flow rate of 1.0 ml/min using a mobile phase that contained acetonitrile and water mixed 50:50, v/v. Internal standard approach was used for quantification. The method shows good linearity (correlation coefficient = 0.9999) with acceptable accuracy, precision and robustness. Three elements were taken into account to measure robustness. Flow rate, mobile phase composition, and pH all have an impact on the response, but only the flow rate which causes a reduction in the concentration of the drug-has a significant impact on the response. Analyst, equipment, and days were taken into consideration for a precision measurement. The analytical procedure had good precision, as seen by the %RSD which is found to be less than 2.0. The proposed method was straightforward, extremely sensitive, exact, and accurate, and it had a retention time of less than 4 minutes, indicating that it is appropriate for daily quality control.