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Updated: Jun 4, 2025

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
19F-PCS measurements on proteins in live mammalian cells
Shu-Li Guo1, Bin-Bin Pan1, Xia-Yan Li1
1State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Abstract:
Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. 19F-PCS is determined in live mammalian cells via dual labelling of the target protein with a paramagnetic tag and a 19F-tag, which is achieved by varied reactivity of solvent exposed cysteines in selection of different types of tags. About 0.1 ppm 19F-PCS can be observed over 20 Å from the paramagnetic center in the live cells.

