Neural Recording Chambers for Long-Term Access to Brain Tissue

We describe a chamber system to perform imaging, electrophysiology, and optogenetic stimulation in awake and anesthetized marmosets. We developed this low-profile chamber design to be able to access the underlying tissue when needed or to leave it sealed for long periods. Such accessibility is useful to maintain chamber clarity as well as perform viral or drug injections at different time points. The chamber is flexible as either optical or electrophysiological recording can be performed in the same chamber by exchanging chamber inserts. The design provides an easy approach to day-to-day stable neural recordings that was developed for marmosets and can be extended to other species.