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Related Experiment Video

Updated: May 8, 2025

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

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Innovative Ink-Based 3D Hydrogel Bioprinted Formulations for Tissue Engineering Applications.

Ana Catarina Sousa1,2,3, Grace Mcdermott4, Fraser Shields4

  • 1Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.

Gels (Basel, Switzerland)
|December 27, 2024
PubMed

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Summary

This study developed a novel alginate-based bioink for 3D bioprinting, enhancing osteogenic models with nano-hydroxyapatite and collagen. The resulting hydrogels promote human bone marrow stem cell viability and osteogenic differentiation for tissue engineering applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Three-dimensional (3D) bioprinting requires advanced biomaterials for creating functional tissue models.
  • Reducing animal testing necessitates innovative in vitro models that accurately mimic native tissue environments.
  • Osteogenic models are crucial for studying bone formation and developing treatments for bone defects.

Purpose of the Study:

  • To develop and characterize a novel alginate-based composite bioink for 3D bioprinting of osteogenic models.
  • To evaluate the cytocompatibility and osteogenic potential of the developed bioink using human bone marrow stem/stromal cells (hBMSCs).
  • To assess the suitability of the 3D bioprinted construct for studying osteogenesis and early tissue maturation in vitro.

Main Methods:

Keywords:
alginatebioinkbioprintingbone regenerationcollagenhuman bone marrow stem/stromal cellshydroxyapatite

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  • Development of a composite bioink using alginate, nano-hydroxyapatite (nHA), type I collagen (Col), and hBMSCs.
  • Extrusion-based 3D bioprinting of hydrogel constructs.
  • Rheological analysis, swelling behavior assessment, LIVE/DEAD viability assay, and PrestoBlue™ cell viability assay.
  • Gene expression analysis of osteogenic markers (SPP1, COL1A2) at 14 days post-printing.

Main Results:

  • The composite bioink exhibited solid-like properties with a superior storage modulus over loss modulus.
  • Incorporation of Col and nHA enhanced hydrogel swelling, promoting cell proliferation.
  • The alginate-nHA-Col hydrogel maintained high hBMSC viability over seven days and showed superior viability compared to other formulations.
  • Gene expression analysis indicated enhanced osteogenic differentiation, with higher SPP1 and COL1A2 expression in hBMSCs within the alginate-nHA-Col bioink.

Conclusions:

  • The developed alginate-nHA-Col composite bioink supports hBMSC viability and promotes osteogenic differentiation.
  • This 3D bioprinted model offers a promising platform for in vitro studies of osteogenesis and bone tissue engineering.
  • The enhanced biomimicry of this model can contribute to reducing animal experimentation and advancing tissue engineering innovations.