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Updated: May 8, 2025
Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
Published on: February 24, 2014
Fluorescence In Situ Hybridization Protocol for Visualization of Oomycetes In Vitro and In Planta
Glen Groben1,2, Catalina Salgado-Salazar3, Jo Anne Crouch4
1United States Department of Agriculture, Agricultural Research Service, Foreign Disease/Weed Science Research Unit, Frederick, MD, USA.
Abstract:
Fluorescence in situ hybridization enables the visualization of organisms in the environment without having to culture them. Here, we describe a FISH protocol to visualize oomycete structures (mycelia, sporangiophores, sporangia, and oospores) directly as well as from colonized plant material. The protocol utilizes organic compounds with low toxicities and does not require a permeabilization step. The protocol was used to visualize impatiens downy mildew Plasmopara destructor sporangiophores colonizing an Impatiens walleriana leaf.
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