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A Chemical Redox Cycling-Based Dual-Mode Biosensor for Self-Powered Photoelectrochemical and Colorimetric Assay of
Xue-Bo Hu1, Yu-Ling Wang1, Jing-Lu Lv1
1College of Chemistry and Chemical Engineering, Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis, Xinyang Normal University, Xinyang 464000, China.
Abstract:
To advance the biological understanding of heat shock protein (HSP) in different types of cancers, it is crucial to achieve its accurate determination. Herein, a dual-mode self-powered photoelectrochemical (PEC) and colorimetric platform was proposed by integrating enzymatic catalysis and a chemical redox cycling amplification strategy. In this system, ascorbic acid (AA), as the signal reporter for PEC and colorimetric assay, can be regenerated during the tris(2-carboxyethyl) phosphine-mediated chemical redox cycling process. For PEC detection, the reproduced electron donor AA could repeatedly combine with holes generated by the Bi2S3/Bi2O3 photoanode to effectively separate the photogenerated electron-hole. Besides, an AA-involved color reaction was evoked during the colorimetric assay to reduce colorless tris(bathophenanthroline) iron(III) to red tris(bathophenanthroline) iron(II). Owing to the ingenious signal amplification strategy, the developed dual-mode assay achieved the PEC and colorimetric determination of HSP90AA1 (one subtype of HSP family) in real samples. It is believed that this work will offer a new strategy to fabricate a dual-mode biosensor, which has great application prospects in the detection of various tumor biomarkers.
