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Updated: May 7, 2025

A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
REMOVAL OF SUBRETINAL GAS AFTER PNEUMATIC RETINOPEXY UNDER AIR USING EXTERNAL MANIPULATION
Lucy Cobbs1, Linnet Rodriguez, John Hinkle
1The Retina Service of Wills Eye Hospital, Wills Eye Physicians-Mid Atlantic Retina, Thomas Jefferson University, Philadelphia, Pennsylvania.
This study demonstrates a safe technique for removing subretinal gas using pars plana vitrectomy (PPV) and scleral depression. The method successfully restored vision and attached the retina without complications, offering a valuable approach for managing retinal detachment.
Area of Science:
- Ophthalmology
- Surgical Techniques
- Retinal Surgery
Background:
- Subretinal gas can cause persistent macula-off retinal detachment.
- Buoyancy of subretinal gas can displace the retina anteriorly, complicating surgical access.
Purpose of the Study:
- To illustrate a technique for subretinal gas removal using pars plana vitrectomy (PPV).
- To demonstrate simultaneous air-fluid exchange and scleral depression for gas extrusion.
Main Methods:
- Pars plana vitrectomy (PPV) was performed to address subretinal gas causing retinal detachment.
- An air-fluid exchange was initiated to stabilize the retina and subretinal gas.
- Scleral depression was employed to guide the subretinal gas towards the retinal break for aspiration.
Main Results:
- One-year postoperative visual acuity was 20/25 with a normal intraocular pressure and attached retina.
- No intraoperative or postoperative complications were reported.
Conclusions:
- A technique combining partial air-fluid exchange and scleral depression effectively stabilizes and extrudes subretinal gas.
- This maneuver, updated with modern vitrectomy equipment, allows excellent control without requiring a retinotomy.
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