Increasing the acquisition speed in oblique plane microscopy via Aliasing

Area of Science:

• Biomedical Imaging
• Microscopy Techniques
• Cell Biology

Background:

• Oblique plane microscopy (OPM), a variant of light-sheet fluorescence microscopy (LSFM), offers rapid volumetric imaging.
• Current OPM methods require small scanning steps to satisfy Nyquist sampling, limiting acquisition speed and increasing sample exposure.

Purpose of the Study:

• To investigate if controlled under-sampling in OPM can accelerate image acquisition without compromising spatial resolution or introducing artifacts.
• To demonstrate the feasibility of leveraging under-sampling for rapid subcellular 3D imaging.

Main Methods:

• Implementation of controlled under-sampling in an OPM system.
• Development of a recovery method to correct aliasing introduced by under-sampling.
• Application of the accelerated OPM technique for imaging mitochondrial dynamics.

Main Results:

• Judicious under-sampling in OPM leads to recoverable aliasing.
• Spatial resolution and image quality are maintained post-recovery.
• Acquisition speed is increased 2-4 fold, depending on optical parameters.

Conclusions:

• Controlled under-sampling is a viable strategy to enhance OPM acquisition speed.
• This method enables rapid subcellular 3D imaging of dynamic biological processes like mitochondrial movement.