Jove
Visualize
Contact Us

Related Concept Videos

Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.2K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.2K
Position-effect Variegation02:32

Position-effect Variegation

6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Light Acquisition02:16

Light Acquisition

8.4K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
8.4K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The HIRA-interacting histone chaperone CABIN1 negatively regulates plant immunity mediated by immune receptor gene SNC1.

Plant physiology·2026
Same author

Aberrant astroglial Kir4.1 activation in the anterior cingulate cortex disrupts neuronal excitability and social behavior.

Acta pharmaceutica Sinica. B·2026
Same author

Hypoxia-induced acidic microenvironment alters cellular pharmacokinetics of gastric cancer in a Mongolian population.

Journal of biomedical research·2026
Same author

The HIRA-interacting histone chaperone CABIN1 negatively regulates plant immunity mediated by immune receptor gene SNC1.

Plant physiology·2026
Same author

Olfactory-to-Entorhinal Network Dysrhythmias Drive Parkinson's Cognitive Impairment Through Frequency-Specific Oscillatory Decoupling.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Multimode neural population coding of diverse innate fear response by mitral and tufted cells.

Cell reports·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

2.8K

Dynamic Chromatin Accessibility and Gene Expression Regulation During Maize Leaf Development.

Yiduo Wang1, Shuai Wang1, Yufeng Wu1

  • 1National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.

Genes
|January 8, 2025
PubMed
Summary
This summary is machine-generated.

Chromatin accessibility dynamics during maize leaf development reveal key regulatory roles in gene expression. This study highlights how accessible chromatin regions influence transcription factor binding and gene regulation across developmental stages.

Keywords:
ATAC-seqchromatin accessibilitygene expression regulationmaize leaf developmenttranscription factors

More Related Videos

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

51.4K

Related Experiment Videos

Last Updated: Jun 3, 2025

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

2.8K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

51.4K

Area of Science:

  • Plant molecular biology
  • Epigenetics
  • Genomics

Background:

  • Chromatin accessibility is vital for transcriptional regulation in maize (Zea mays) leaf development.
  • The precise role of chromatin accessibility in gene expression across different developmental stages is not fully understood.

Purpose of the Study:

  • To investigate the dynamics of chromatin accessibility during maize leaf development.
  • To understand the influence of chromatin accessibility on genome-wide gene expression at BBCH_11, BBCH_13, and BBCH_17 stages.

Main Methods:

  • Maize leaves were analyzed at BBCH_11, BBCH_13, and BBCH_17 stages.
  • Chromatin accessibility was assessed using ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing).
  • Gene expression was profiled using RNA-seq (RNA sequencing), followed by integrated data analysis.

Main Results:

  • Identified thousands of accessible chromatin regions (ACRs) across developmental stages, with a portion near transcription start sites (TSSs).
  • Demonstrated that the number and intensity of ACRs significantly impact gene expression levels.
  • Motif analysis revealed transcription factors involved in leaf development and identified genes with divergent regulation patterns.

Conclusions:

  • Chromatin accessibility is crucial for spatial and temporal gene expression regulation in maize leaf development.
  • Modulation of transcription factor binding by accessible chromatin influences gene expression.
  • This research offers new insights into chromatin-mediated gene expression and maize leaf development.