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Published on: November 23, 2016
Anticholinesterase Activity and Bioactive Compound Profiling of Six Hop (Humulus lupulus L.) Varieties.
Bartłomiej Sagan1, Bogusław Czerny2, Anna Stasiłowicz-Krzemień3
1Department of Neurosurgery and Pediatric Neurosurgery, Pomeranian Medical University Hospital No. 1 in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, Poland.
This study reveals significant variation in bioactive compounds across six hop varieties, with Aurora and Zwiegniowski showing the highest concentrations of key compounds. Aurora demonstrated potent antioxidant and enzyme inhibitory activities, suggesting pharmaceutical potential.
Area of Science:
- Phytochemistry
- Pharmacology
- Biotechnology
Background:
- Hops (Humulus lupulus L.) are known for brewing and possess pharmacological properties due to bioactive compounds.
- Significant variability exists in the chemical composition and biological activities among different hop varieties.
- Understanding these variations is crucial for exploring pharmaceutical and nutraceutical applications.
Purpose of the Study:
- To investigate the chemical composition and biological activities of extracts from six hop varieties.
- To quantify xanthohumol, lupulone, and total phenolic content (TPC) using HPLC and TPC analysis.
- To evaluate antioxidant, enzyme inhibitory, and metal ion chelating activities of hop extracts.
Main Methods:
- Six hop varieties were analyzed for bioactive compounds using High-Performance Liquid Chromatography (HPLC).
- Total Phenolic Content (TPC) was determined using spectrophotometric methods.
- Biological activities including antioxidant (DPPH, ABTS, CUPRAC, FRAP), enzyme inhibition (AChE, BChE, tyrosinase, hyaluronidase), and metal ion chelation were assessed.
Main Results:
- Hop varieties showed significant differences in xanthohumol, lupulone, and TPC concentrations.
- Aurora exhibited the highest xanthohumol (0.665 mg/g) and TPC (22.47 mg GAE/g).
- Aurora and Oregon Fuggle displayed potent antioxidant activities, while Aurora showed strong inhibition of acetylcholinesterase, butyrylcholinesterase, tyrosinase, and hyaluronidase.
Conclusions:
- Genetic and environmental factors significantly influence the bioactive profiles and biological activities of hop varieties.
- Aurora hop extract demonstrates considerable potential for pharmaceutical and nutraceutical applications due to its rich bioactive compounds and potent activities.
- Further research is necessary to elucidate the complex interactions among hop cone components for comprehensive application development.
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