Surface-Enhanced Raman Spectroscopy for the Detection of Reactive Oxygen Species.

Area of Science:

• Analytical Chemistry
• Biochemistry
• Spectroscopy

Background:

• Reactive oxygen species (ROS) are crucial in biological and industrial processes but challenging to detect due to their transient nature.
• Accurate detection of ROS is vital for understanding cell signaling, disease, aging, and industrial applications.
• Existing detection methods face limitations in sensitivity, selectivity, and real-time monitoring capabilities.

Purpose of the Study:

• To provide a comprehensive review of Surface-Enhanced Raman Spectroscopy (SERS) for detecting reactive oxygen species (ROS).
• To critically evaluate SERS principles, sensing mechanisms, and applications for ROS monitoring.
• To discuss the development of selective SERS probes for various ROS and identify future research directions.

Main Methods:

• Review of existing literature on SERS applications for ROS detection.
• Analysis of SERS principles and ROS sensing mechanisms.
• Compilation and discussion of selective SERS probes for superoxide, hydroxyl radicals, nitric oxide, peroxynitrite, and hypochlorite.

Main Results:

• SERS is a highly sensitive and selective technique suitable for real-time, in situ ROS detection.
• Various SERS probes have been developed for specific ROS, enabling direct and indirect monitoring.
• SERS facilitates the study of ROS-involved electrochemical and biochemical events with high resolution.

Conclusions:

• SERS presents a powerful platform for advancing ROS analysis in diverse fields.
• Further development of SERS probes and methodologies will enhance ROS detection capabilities.
• Addressing technical challenges in SERS for ROS analysis is crucial for future research and applications.