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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: Jun 3, 2025

Erythrocyte Sedimentation Rate: A Physics-Driven Characterization in a Medical Context

Erythrocyte Sedimentation Rate: A Physics-Driven Characterization in a Medical Context

Published on: March 24, 2023

Preanalytical Sedimentation Effects on Hematological Tests.

Kenneth Gard¹, Ian L Gunsolus²

¹Regions Hospital Pathology Lab, Client Services, St. Paul, MN, United States.

The Journal of Applied Laboratory Medicine

|January 9, 2025

Summary

Inaccurate blood test results due to red blood cell sedimentation can be prevented. Inverting blood collection syringes before transfer to tubes ensures accurate complete blood count (CBC) analysis.

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Last Updated: Jun 3, 2025

Erythrocyte Sedimentation Rate: A Physics-Driven Characterization in a Medical Context

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Published on: March 24, 2023

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry

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Published on: January 12, 2018

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Published on: June 4, 2015

Area of Science:

Hematology
Clinical Pathology
Laboratory Medicine

Background:

Blood specimen errors, often attributed to contamination, can lead to inaccurate hemoglobin levels.
When contamination is ruled out, specimen handling errors like sedimentation may cause variations.
Proper evaluation of blood samples between collection and testing is crucial.

Purpose of the Study:

To investigate the impact of blood sedimentation on complete blood count (CBC) results.
To evaluate the effectiveness of specimen handling techniques in preventing sedimentation-related errors.
To improve the accuracy of CBC testing in hospital settings.

Main Methods:

Blood specimens were collected in syringes and allowed to stand vertically for 10 minutes.
Syringes were divided into top and bottom halves, with some samples inverted before division.
Samples were analyzed for complete blood count (CBC) parameters.

Main Results:

Blood sedimentation significantly impacts CBC results when specimens are not immediately transferred.
Inverting collected blood specimens prior to transfer effectively mitigates variations caused by sedimentation.
Proper mixing is essential to ensure sample homogeneity.

Conclusions:

Specimen handling after blood collection is critical for accurate laboratory results.
Erythrocyte sedimentation can cause erroneous CBC findings if not addressed.
Inverting syringes before transferring blood to collection tubes ensures sample uniformity and reliable results.