A novel 3D-printed sample preparation method for benzodiazepine quantification in human serum.

Area of Science:

• Analytical Chemistry
• Materials Science
• Forensic Toxicology

Background:

• Benzodiazepine abuse is a major public health issue.
• Existing sample preparation methods are complex, require large volumes, and use excessive solvents.
• There is a need for more efficient and sustainable analytical techniques.

Purpose of the Study:

• To develop and validate a novel, efficient sample preparation method for benzodiazepine analysis.
• To utilize 3D-printed sorbent devices for extracting benzodiazepines from human serum.
• To overcome the limitations of traditional sample preparation techniques.

Main Methods:

• Fabrication of 3D-printed sorbent devices using a C18-modified silica thermoplastic composite.
• Optimization and validation of the extraction method according to ICH guidelines.
• Application of the method for quantitative benzodiazepine analysis in human serum using LC-MS.

Main Results:

• The 3D-printed devices showed high efficiency in extracting benzodiazepines.
• The method required minimal sample and solvent volumes.
• Protein precipitation, evaporation, and reconstitution steps were eliminated, simplifying the workflow.

Conclusions:

• The novel 3D-printed sorbent device offers a more efficient and environmentally friendly approach for benzodiazepine analysis.
• The 3D printing technology allows for customizable sorbent devices for various analytes and matrices.
• This method, combined with LC-MS, provides a sensitive tool for clinical and toxicological monitoring of benzodiazepines.