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Related Experiment Video

Updated: Jun 2, 2025

Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology
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Serum assisted PD-L1 aptamer screening for improving its stability.

Yu Zhou1, Haozhe Zhang2, Yujing Ding2

  • 1School of Public Health, Jining Medical University, Jining, 272067, People's Republic of China.

Scientific Reports
|January 13, 2025
PubMed
Summary

A new aptamer selection strategy significantly improves aptamer stability and specificity for PD-L1 detection. This enhanced aptamer shows promise for cancer diagnosis and treatment applications.

Keywords:
Aptamer selectionAptamer stabilityPD-L1Real capture-SELEX strategySerum

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers offer potential for disease diagnosis and targeted therapy.
  • Limited aptamer stability and short half-life restrict their clinical use.

Purpose of the Study:

  • To develop a real sample-assisted capture-SELEX strategy to enhance aptamer stability.
  • To select a highly stable and specific aptamer against PD-L1.

Main Methods:

  • A novel real sample-assisted capture-SELEX strategy was employed.
  • Aptamers targeting PD-L1 were selected and characterized.
  • Aptamer stability was assessed in human serum under physiological conditions.

Main Results:

  • The developed strategy yielded Apt-S1 with superior binding affinity and specificity for PD-L1 compared to Apt-A2 (traditional SELEX).
  • Apt-S1 demonstrated enhanced conformational changes upon PD-L1 binding, indicating suitability as a biorecognition element.
  • Apt-S1 exhibited significantly lower degradation rates in human serum, suggesting improved stability attributed to pre-adaptation to serum nucleases.

Conclusions:

  • The real sample-assisted capture-SELEX strategy effectively enhances aptamer stability and specificity.
  • Apt-S1 is a promising candidate for clinical applications in PD-L1-targeted cancer diagnosis and therapy.
  • This method offers advantages over traditional SELEX for developing robust aptamers.