Freeze-dried plasma: Hemostasis and biophysical analyses for damage control resuscitation
Aron A Shoara1,2,3,4, Kanwal Singh2,3,4,5, Henry T Peng5
1Department of Surgery, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
Transfusion
|January 14, 2025
Summary
Freeze-dried plasma (FDP) shows comparable hemostatic function and stability to fresh plasma. This makes FDP a viable alternative for prehospital resuscitation, overcoming logistical challenges.
Area of Science:
- Biochemistry
- Hematology
- Biophysics
Background:
- Effective hemorrhage protocols require immediate hemostatic resuscitation for hemorrhagic shock.
- Prehospital resuscitation with blood products can improve outcomes but faces logistical hurdles.
- Freeze-dried plasma (FDP) offers a potential solution to storage and transport challenges.
Purpose of the Study:
- To compare the functional and structural properties of freeze-dried plasma (FDP) with fresh plasma controls.
- To assess the hemostatic functionality and protein stability of FDP using advanced biophysical techniques.
Main Methods:
- Hemostatic assays were performed.
- Advanced biophysical techniques including calorimetry, infrared spectroscopy, dynamic light scattering, and biolayer interferometry were utilized.
- Functional and structural properties of FDP were compared to fresh plasma.
Main Results:
- FDP demonstrated largely comparable clot formation and coagulation parameters to fresh plasma.
- No significant change in moisture content was observed in FDP after 6-month storage.
- Biophysical analyses showed comparable stability and ligand-binding affinities for FDP during transfusion.
Conclusions:
- FDP exhibits hemostatic functionality and protein stability comparable to fresh plasma.
- FDP is a viable alternative for damage control resuscitation, especially in prehospital and remote settings.
- FDP offers significant logistical and storage advantages over conventional plasma.
Keywords:
blood productscoagulopathydamage control resuscitationfreeze‐dried plasmahemorrhagemicrocalorimetrytraumaMore Related Videos
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