Advanced LC-IMS-MS Protocol for Holistic Metabolite Analysis in Wine and Grape Samples

Area of Science:

• Analytical Chemistry
• Metabolomics
• Food Science

Background:

• Metabolomics aims for holistic study of the metabolome in biological samples.
• Complex samples like wine require robust, sensitive, and specific analytical techniques for metabolite analysis.
• Hardware advancements in hyphenated instruments have improved multi-class metabolite analysis.

Purpose of the Study:

• To describe a liquid chromatography-ion mobility spectrometry-mass spectrometry (LC-IMS-MS) protocol for wine and grape metabolomics.
• To highlight the utility of ion mobility spectrometry (IMS) data for metabolite annotation.
• To enable comprehensive analysis of complex biological samples like wine.

Main Methods:

• Development and application of a hyphenated LC-IMS-MS analytical protocol.
• Analysis of metabolites in wine and grape samples.
• Utilizing ion mobility spectrometry data for enhanced metabolite identification.

Main Results:

• The described LC-IMS-MS protocol allows for robust, sensitive, and specific analysis of wine and grape metabolomes.
• Ion mobility spectrometry data significantly aids in the annotation of detected metabolites.
• The method is suitable for analyzing complex samples with diverse chemical classes and concentration ranges.

Conclusions:

• LC-IMS-MS is a powerful approach for comprehensive metabolomics in complex matrices like wine.
• The integration of IMS data improves the accuracy and reliability of metabolite annotation.
• This protocol facilitates a deeper understanding of the metabolome in grapes and wine.